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anti ps79 acc1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti ps79 acc1
Anti Ps79 Acc1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acc1+ps79/Phospho-Acetyl-CoA+Carboxylase+(Ser79)+Antibody/pmc12182294-221-43-45
Average 96 stars, based on 1 article reviews
anti ps79 acc1 - by Bioz Stars, 2026-09
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acc1 ps79  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc acc1 ps79
Immunoprecipitation or immunoblot analyses were performed with the indicated antibodies. ( A ) Huh7 cells transfected with Flag–KHK-A were pretreated with SB203580 (10 μM), SP600125 (25 μM), U0126 (20 μM), or compound C (5 μM) before hypoxia stimulation for 6 hours in the presence of CQ (10 μM). ( B ) Huh7 cells expressing Flag–KHK-C or Flag–KHK-A were cotransfected with HA-AMPKα and treated with or without hypoxia for 6 hours or H 2 O 2 (0.5 mM) for 1 hour in the presence of the lysosome inhibitor CQ (10 μM). ( C ) Huh7 cells transfected with Flag–KHK-A were pretreated with or without compound C (5 μM) for 30 min before hypoxia stimulation for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). <t>ACC1,</t> acetyl-CoA carboxylase 1. ( D ) WT and AMPKα1/2 double-knockout (DKO) mouse embryonic fibroblasts (MEFs) infected with lentivirus expressing Flag–KHK-A were treated with or without A769662 (0.5 mM) for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ( E and F ) Bacterially purified GST–KHK-A, GST–KHK-C (E), or GST–KHK-A S80A (F) was incubated with or without bacterially purified active AMPK in the presence of [γ- 32 P]ATP. Autoradiography and immunoblot analyses were performed. ( G ) Huh7 cells expressing WT Flag–KHK-A, Flag–KHK-C, or Flag–KHK-A S80A were treated with or without hypoxia for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ( H ) WT and AMPKα1/2 DKO MEFs were cultured with or without hypoxia for 6 hours. ( I ) An in vitro AMPK kinase assay was performed by mixing bacterially purified WT GST–KHK-A, GST–KHK-A S80A, or GST–KHK-C on glutathione agarose beads with or without purified active AMPK in the presence of AMP and ATP for 1 hour. The glutathione agarose beads were then washed and incubated with purified His-p62 for a GST pulldown analysis. ( J ) Huh7 cells expressing Flag–KHK-A, Flag–KHK-C, or Flag–KHK-A S80A were treated with or without hypoxia for 4 hours in the presence of the lysosome inhibitor CQ (10 μM). ( K ) Huh7 cells expressing Flag–KHK-A and HA-AMPKα were pretreated with N -acetyl- l -cysteine (NAC) (5 mM) or Trolox (100 μM) for 30 min before hypoxia stimulation for 6 hours. ( L ) Huh7 cells expressing Flag–KHK-A were pretreated with NAC (5 mM) or Trolox (100 μM) for 30 min before hypoxia stimulation for 6 hours in the presence of the lysosome inhibitor CQ (10 μM).
Acc1 Ps79, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acc1+ps79/Phospho-Acetyl-CoA+Carboxylase+(Ser79)+Rabbit+mAb/pmc06482012-166-28-50
Average 96 stars, based on 1 article reviews
acc1 ps79 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

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Immunoprecipitation or immunoblot analyses were performed with the indicated antibodies. ( A ) Huh7 cells transfected with Flag–KHK-A were pretreated with SB203580 (10 μM), SP600125 (25 μM), U0126 (20 μM), or compound C (5 μM) before hypoxia stimulation for 6 hours in the presence of CQ (10 μM). ( B ) Huh7 cells expressing Flag–KHK-C or Flag–KHK-A were cotransfected with HA-AMPKα and treated with or without hypoxia for 6 hours or H 2 O 2 (0.5 mM) for 1 hour in the presence of the lysosome inhibitor CQ (10 μM). ( C ) Huh7 cells transfected with Flag–KHK-A were pretreated with or without compound C (5 μM) for 30 min before hypoxia stimulation for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ACC1, acetyl-CoA carboxylase 1. ( D ) WT and AMPKα1/2 double-knockout (DKO) mouse embryonic fibroblasts (MEFs) infected with lentivirus expressing Flag–KHK-A were treated with or without A769662 (0.5 mM) for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ( E and F ) Bacterially purified GST–KHK-A, GST–KHK-C (E), or GST–KHK-A S80A (F) was incubated with or without bacterially purified active AMPK in the presence of [γ- 32 P]ATP. Autoradiography and immunoblot analyses were performed. ( G ) Huh7 cells expressing WT Flag–KHK-A, Flag–KHK-C, or Flag–KHK-A S80A were treated with or without hypoxia for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ( H ) WT and AMPKα1/2 DKO MEFs were cultured with or without hypoxia for 6 hours. ( I ) An in vitro AMPK kinase assay was performed by mixing bacterially purified WT GST–KHK-A, GST–KHK-A S80A, or GST–KHK-C on glutathione agarose beads with or without purified active AMPK in the presence of AMP and ATP for 1 hour. The glutathione agarose beads were then washed and incubated with purified His-p62 for a GST pulldown analysis. ( J ) Huh7 cells expressing Flag–KHK-A, Flag–KHK-C, or Flag–KHK-A S80A were treated with or without hypoxia for 4 hours in the presence of the lysosome inhibitor CQ (10 μM). ( K ) Huh7 cells expressing Flag–KHK-A and HA-AMPKα were pretreated with N -acetyl- l -cysteine (NAC) (5 mM) or Trolox (100 μM) for 30 min before hypoxia stimulation for 6 hours. ( L ) Huh7 cells expressing Flag–KHK-A were pretreated with NAC (5 mM) or Trolox (100 μM) for 30 min before hypoxia stimulation for 6 hours in the presence of the lysosome inhibitor CQ (10 μM).

Journal: Science Advances

Article Title: The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62

doi: 10.1126/sciadv.aav4570

Figure Lengend Snippet: Immunoprecipitation or immunoblot analyses were performed with the indicated antibodies. ( A ) Huh7 cells transfected with Flag–KHK-A were pretreated with SB203580 (10 μM), SP600125 (25 μM), U0126 (20 μM), or compound C (5 μM) before hypoxia stimulation for 6 hours in the presence of CQ (10 μM). ( B ) Huh7 cells expressing Flag–KHK-C or Flag–KHK-A were cotransfected with HA-AMPKα and treated with or without hypoxia for 6 hours or H 2 O 2 (0.5 mM) for 1 hour in the presence of the lysosome inhibitor CQ (10 μM). ( C ) Huh7 cells transfected with Flag–KHK-A were pretreated with or without compound C (5 μM) for 30 min before hypoxia stimulation for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ACC1, acetyl-CoA carboxylase 1. ( D ) WT and AMPKα1/2 double-knockout (DKO) mouse embryonic fibroblasts (MEFs) infected with lentivirus expressing Flag–KHK-A were treated with or without A769662 (0.5 mM) for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ( E and F ) Bacterially purified GST–KHK-A, GST–KHK-C (E), or GST–KHK-A S80A (F) was incubated with or without bacterially purified active AMPK in the presence of [γ- 32 P]ATP. Autoradiography and immunoblot analyses were performed. ( G ) Huh7 cells expressing WT Flag–KHK-A, Flag–KHK-C, or Flag–KHK-A S80A were treated with or without hypoxia for 6 hours in the presence of the lysosome inhibitor CQ (10 μM). ( H ) WT and AMPKα1/2 DKO MEFs were cultured with or without hypoxia for 6 hours. ( I ) An in vitro AMPK kinase assay was performed by mixing bacterially purified WT GST–KHK-A, GST–KHK-A S80A, or GST–KHK-C on glutathione agarose beads with or without purified active AMPK in the presence of AMP and ATP for 1 hour. The glutathione agarose beads were then washed and incubated with purified His-p62 for a GST pulldown analysis. ( J ) Huh7 cells expressing Flag–KHK-A, Flag–KHK-C, or Flag–KHK-A S80A were treated with or without hypoxia for 4 hours in the presence of the lysosome inhibitor CQ (10 μM). ( K ) Huh7 cells expressing Flag–KHK-A and HA-AMPKα were pretreated with N -acetyl- l -cysteine (NAC) (5 mM) or Trolox (100 μM) for 30 min before hypoxia stimulation for 6 hours. ( L ) Huh7 cells expressing Flag–KHK-A were pretreated with NAC (5 mM) or Trolox (100 μM) for 30 min before hypoxia stimulation for 6 hours in the presence of the lysosome inhibitor CQ (10 μM).

Article Snippet: Rabbit antibodies against mitogen-activated protein kinase-activated protein kinase 2 (MAPK/APK2) (#3042), MAPK/APK2 pT222 (#3316), c-Jun (#9165), c-Jun pS73 (#9164), Trx1 (#2429), AMPKα (#5831), AMPKα pT172 (#2535), ACC1 (#3676), ACC1 pS79 (#11818), LC3B (#3868), cleaved PARP (Asp 214 ) (#9541), and anti-rabbit IgG conformation-specific secondary antibody (L27A9) (#3678) were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: Immunoprecipitation, Western Blot, Transfection, Expressing, Double Knockout, Infection, Purification, Incubation, Autoradiography, Cell Culture, In Vitro, Kinase Assay